Investigation of potential antiurolithiatic activity and in silico docking studies of Karpura shilajit

In this present study, Karpura shilajit, an Ayurvedic herbo-mineral, currently used in the clinical practice for various ailments, was investigated chemically and pharmacologically.To screen anti-lithiatic activity of shilajit against sodium oxalate (70 mg/kg, i.p) 7 days vitro by nucleation-aggregation assay. Cystone (500 p.o.)used as a standard drug study.Nephrolithiasis may also be associated with nephrocalcinosis, i.e., crystal depositions tubular lumen and/or interstitium, entity which suggests specific pathological processes. Preliminary Phytochemical screening resulted presence alkaloids, flavonoids, saponins, carbohydrates, terpenoids steroids. significantly restored creatinine, urea, uric acid, calcium, total protein, potassium levels insodium induced urolithiasis model. Histopathological examination further confirmed induction long standing hypercalciuria or hyperoxaluria, conversely to nephrocalcinosis sections kidney treated oxalate. treatment normalized biochemical renal stone markers experimental rats showed better vivo models. had shown significant effect on urine volume, pH, excretion, levels.